The Dutch League champions, who trailed 2-1 from last week’s first leg in Turin, turned on the style to secure a remarkable 3-1 comeback and continue their impressive home record in European competition.
This turbulent victory for head coach Peter Bosz made it a staggering 13-match unbeaten home record in Europe, with PSV’s success over Juventus their fourth win on the bounce.
Veteran striker Ivan Perisic broke the deadlock on 54 minutes, bagging his sixth career goal against the Bianconeri to level the play-off aggregate scores.
Timothy Weah blasted in an equaliser 10 minutes later, which was only awarded following a VAR review.
Ismael Saibari restored the lead for the hosts with just over quarter-of-an-hour remaining. Flamingo’s decisive goal arrived eight minutes into extra time to secure an outstanding 4-3 aggregate comeback.
The Philips Stadion was an intimidating cauldron for Thiago Motta’s troops, who battled throughout the dull first half. But they lost a key player to injury after just 10 minutes.
Renato Veiga, who had won every match he had played 90 minutes for Juve, went off injured early on and the club’s “lucky charm” was gone.
Without Chelsea loanee Veiga, Andrea Cambiaso on his return from injury sent on as a replacement, the visitors were simply not the formidable force they had been over the past four games.
Juve had been on a roll with four consecutive victories, including the first leg 2-1 success over PSV in Turin, with Veiga playing a significant role.
This was yet another game of two halves for the Bianconeri, like their Derby D’Italia win over Inter Milan. The listless opening 45 minutes without a goal left both sets of fans subdued inside the stadium.
Yet the scintillating second-half exploded with pure drama and four fabulous goals. Motta’s men were outplayed to taste their first reversal since last month’s loss to Napoli in Serie A, with neither hot-shot forward Kolo Muani nor his replacement Dusan Vlahovic able to make much of an impact.
Noa Lang kept tormenting the Juve defense with his fancy footwork and swift breaks on the left flank. It was only a question of time as to when he would create the opening goal.
Lang’s darting run from the halfway line saw him slip past opponents like a hot knife through butter, picking out Croatia international Perisic to thump the ball into the bottom corner past 'keeper Michele Di Gregorio.
Perisic had found the back of the net in the first leg, and bagged four goals against Juventus when he played for Inter.
It looked like the 36-year-old would notch his third Champions League goal this term, but his sizzling shot was saved. PSV skipper Luuk de Jong also came close, only for his header to be cleared off the line.
With PSV bombarding the Juve penalty area, it required resolute defending to stave off the relentless danger.
Weah’s sensational thunderbolt from outside the area found the bottom left corner following a set piece, but there was a delay while the referee checked the VAR screen before pointing to the middle.
With Juve leading overall, it took tame defending to allow PSV back into the contest. De Long was unable to line up a clean shot, and Saibari was quickest to the loose ball that he dispatched into the top right corner. The home fans went wild as it was game on after 74 minutes.
There was further joy for the PSV fans when Flamingo found the back of the net from close range for what proved to be the winner on 98 minutes, to deliver the knockout blow for Juventus.
The Bianconeri will feel the pinch from not scooping potential money earned from reaching the last 16, and possibly progressing further in Europe’s top club competition.
PSV ended their recent poor run of results, while Juve’s were expected to waltz through this clash having strung together a four-match winning run. Although those games all featured Veiga, and his absence after an early injury was felt after Juventus whimpered out of Europe.