Juve boss Spalletti is now missing 11 key players who are away on international duty, with midfielder Khephren Thuram having been called up to cover for injured Real Madrid star Eduardo Camavinga.
France head coach Didier Deschamps put Thuram onto the standby list rather than the final squad that tackles the World Cup qualifiers.
The two-time world champions, who host Ukraine before visiting Azerbaijan, are expected to book their berth for next year's World Cup finals. Yet they will be without the versatile Camavinga because of his hamstring injury.
Thuram, who made two appearances for the Les Bleus during the previous international break, was a surprise omission by Deschamps. He is unlikely to feature against Ukraine at the Parc des Princes in Paris, apart from appearing on the bench, as he is a late draft to the France squad. Although could play in Azerbaijan.
Maybe Thuram was overlooked because confidence was extremely low at Juventus under former manager Igor Tudor, whose one-dimensional tactics were visibly affecting players. Thuram also showed some physical struggles in recent weeks, but he is favored by new boss Spalletti.
Spalletti's stuttering Bianconeri start
Spalletti's start to life at Juventus has shown some signs of improvement, although his new team have only mustered three goals in as many games. A slender 2-1 success at Cremonese was a game of two halves, with the defensive vulnerabilities apparent.
Despite kicking off with a hard-fought victory, Spalletti has since seen his side draw at home against Sporting Lisbon and Torino.
Spalletti stuck to Tudor's tactics of three defenders, as he was aware the team has been set up for this formation, but in the trio of matches they looked toothless, lacked creativity and were shaky in defense.
These problems need ironing out over the two-week international break, with Spalletti expected to transform the fortunes of the fallen Italian giants by implementing a new tactical approach.
His ill-fated spell as Italy manager only nosedived when he started played three defenders and a loan striker, the same as Tudor implemented with little success at Juventus. Versatile Spalletti is renowned for setting up his side to win matches, so some similar formations to his success spell at Napoli is expected when the Bianconeri return to action.
As Spalletti built his Scudetto-winnning Napoli team around a traditional center forward and whizzy winger, it is anticipated that he can replicate this at Juventus. Dusan Vlahovic and Kenan Yildiz, who could both leave in the January transfer window as contract talks have stalled, would be ideal scenario for Spalletti's 4-3-3 tactics that made Napoli highly competitive and a joy to watch.
Juventus have a silverware goal
There are a plethora of fringe players who could be given a chance to shine over the coming weeks if Spalletti decides to shake things up and rotate his squad. Juve host Udinese in the Coppa Italia, but a reserve side is highly improbable to be fielded as it is the club's only realistic chance to win silverware this season.
Juve's Champions League campaign is in tatters at the halfway stage of the league phase, having yet to pick up maximum points. They have four more matches to leap up the table in order to qualify for the knockout stages, and will tackle opponents that on paper they are expected to beat.
Their objective to finish in the Serie A top four, which secures Champions League soccer next term, is viable. They sit seventh after 11 games, and only lie six points behind table-topping Inter Milan.
AC Milan, AS Roma, Bologna, Como, Inter and Napoli are their current rivals for a coveted top four place at the end of the season. However, Atalanta could soon be added to the mix after they appointed former Fiorentina manager Raffaele Palladino, a former Juventus player who was sounded out by the Turin titans to one day take over the Bianconeri hot seat.
Spalletti gave his squad four days off from training to recharge their batteries while he explored various tactical approaches in a bid to get Juve's season back on track.
This two-week break offers Spalletti the chance to evaluate his squad, with 11 players away on international duty, and come up with solutions to make Juventus highly competitive.
Goal-getting keeps eluding Juve
The massive problem is that only Vlahovic is managing to score goals this season, with Jonathan David having not found the back of the net since his Serie A debut in August and Lois Openda still without a goal since arriving on deadline day as a surprise transfer loanee from RB Leipzig.
Both David and Openda, who have recently been poor for club and country, away on international duty leaves Spalletti needing to urgently work out where goals can come from.
Weston McKennie, who was overlooked by the USA for their friendly matches, could be utilized upfront during training and maybe become one of three attackers when Juve return to competitive action. Since Tudor was shown the door, McKennie has been one of the Bianconeri's best players.
Spalletti will undertake training without key international players Vasilije Adzic (Montenegro), Andrea Cambiaso (Italy), Francisco Conceicao (Portugal), David (Canada), Filip Kostic (Serbia), Manuel Locatelli (Italy), Openda (Belgium), Thuram (France), Vlahovic (Serbia), Yildiz (Turkey) and Edon Zhegrova (Kosovo).
Yet there are plenty of players who could step up to the mark under Spalletti. A poor performance from defender Daniele Rugani, who was replaced at half-time during the scoreless Derby della Mole, will have done the former Italy international no favors.